Primary Template-directed Amplification (PTA)
As the anchor of BioSkryb technology, PTA delivers highly uniform, low-error whole-genome amplification so every downstream analysis starts with the most faithful view of biology.
Why PTA is the anchor
PTA preserves allelic balance, minimizes amplification bias, and maintains true signal at single-cell resolution. The chemistry was purpose-built to eliminate common WGA artifacts and provide confidence in every base you call.
- Ultra-low error rates that maintain true SNV signal and allele balance.
- Consistent genome coverage with minimized dropouts across GC extremes.
- Reproducible performance that scales from discovery to translational programs.
Coverage that stays complete
PTA evenly amplifies single-cell genomes, enabling sensitive detection of point mutations, copy number variation, and structural events without coverage cliffs.
- Uniform amplification supports accurate CNV calling and SV detection.
- High-complexity libraries that retain rare variants and subclonal signals.
- Compatibility with leading sequencing platforms for flexible throughput.
Built for end-to-end workflows
PTA integrates seamlessly with your preferred single-cell isolation and library prep methods so projects can move quickly from sample to interpretable data.
- Flexible compatibility with FACS, microfluidics, and low-input samples.
- High-concordance data that feeds AI-enabled analysis pipelines.
- Optimized kits and protocols supported by BioSkryb experts.
Ready to talk?
Contact us Discuss how PTA can anchor your next single-cell study.